WebNov 24, 2024 · We evaluated two conditional (Csf1r-Cre and LysM-Cre) and two inducible [CX 3 CR1-estrogen receptor-Cre (ERCre) and CD68-rtTA] lines. We assessed Cre … WebSep 2, 2024 · However, a recent study proposed that osteoclasts may live more than 6 months by acquiring new nuclei from circulating precursors based on the following observation; when parabionts of Csf1r-Cre: Rosa26LSL-YFP mouse (osteoclast precursors labeled with YFP) and Csf1r-Cre: Rosa26LSL-tdTomato mouse (osteoclast precursors …
Osteoclast biology in the single-cell era Inflammation and ...
Web021212 B6.Cg- Csf1r tm1.2Jwp /J These Csf1r fl/fl floxed mutant mice possess loxP sites flanking exon 5 of the colony stimulating factor 1 receptor ( Csf1r ) gene and may be useful in studying the role of CSF1R in development. WebOct 1, 2024 · Lyve1Cre targets lymphatic endothelial cells and VAMs, and Lyz2Cre targets many myeloid cell populations, including macrophages, monocytes, and neutrophils, while Csf1rCre targets macrophages and erythromyeloid progenitors, the precursors of yolk-sac–derived tissue-resident macrophages. how much protein for 165 lb man
c-MAF–dependent perivascular macrophages regulate diet-induced ...
WebCre-ERT2在无Tamoxifen诱导的情况下,在细胞质内处于无活性状态;当Tamoxifen诱导后,Tamoxifen的代谢产物4-OHT(雌激素类似物)与ERT结合,可使Cre-ERT2进核发 … Webwith inducible transgenic Csf1R-CreER mice to permanently label yolk sac progenitors. Csf1r is active in yolk sac progenitor cells at gestational day 8-9 (gd8-9), thus a 4hydroxytamoxifen (4-OHT) pulse at gd8.5 will label yolk sac-derived macrophages prior to their migration out of the yolk sac (Sasmono et al., 2003). Web例如Christian Schulz等利用组成性表达Cre的Csf1r-cre鼠及诱导性表达Cre的Csf1r-MeriCreMer与Rosa26-LSL-YFP小鼠交配,获得在Csf1r基因表达的细胞中表达YFP荧光 … how much protein for 140 lb woman