Exo-fecttm sirna/mirna transfection kit

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August undefined, 2024

WebAmbion makes this solution hybridization assay available as the mirVana miRNA Detection Kit. We also offer the mirVana miRNA Probe Construction Kit, for the easy preparation of short RNA probes for this assay as well as for Northern analysis and in situ hybridization. Ordering Information WebHiPerFect Transfection Reagent was developed for highly efficient transfection of eukaryotic cells with siRNA, and is ideally suited to transfection of miRNA mimics or inhibitors. Procedure HiPerFect Transfection Reagent is provided as a ready-to-use solution - just add the reagent to your diluted siRNA/miRNA, mix, incubate, and pipet the ...

Detecting miRNA and siRNA Thermo Fisher Scientific - US

WebThe Silencer® siRNA Labeling Kits are the perfect complement to the Silencer siRNA Construction Kit, but can also be used to label chemically synthesized siRNAs. Two labeling kits are available: Cy3 and FAM. Each kit comes with all … WebJan 13, 2024 · It has been described that miR-375 is inversely associated with EMT in cancerous patients and can effectively inhibit invasion and migration of tumor cells. Here, we investigate whether miR-375... fbb alessandra alvez

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WebThe Exo-Fect siRNA/miRNA Transfection Kit workflow is quick and easy—complete in 1.5 hours and with only 15-minutes of hands-on time. The workflow consists of two steps: … With a wealth of options for gene delivery and expression, SBI has the gene … EZ-Genotyping Kit; CLOuD9 Gene Expression Regulation System; … For the fastest and most sensitive antibody-based quantitation, our new ExoELISA … Use our SeraMir family of products for qPCR profiling of exosmal miRNA, save … SBI’s ExoGlow-NTA Fluorescent Labeling Kit delivers highly accurate EV … WebsiRNA transfection is a laboratory technique used to deliver small interfering RNA (siRNA) molecules into cells to silence specific genes or to study gene function. The siRNA molecules are designed to bind to and degrade the messenger RNA (mRNA) of the target gene, resulting in the downregulation or knockdown of gene expression. WebTriFECTa RNAi Kit With the TriFECTa Kit, you receive all of the duplexes you need for successful RNA knockdown. These include: 3 Predesigned DsiRNAs that are specific for a single target gene 3 control DsiRNAs for optimizing your RNAi experimental setup: TYE 563 Transfection Control DsiRNA, 1 nmol HPRT-S1 Positive Control DsiRNA, 1 nmol fbb ais

DsiRNAs and TriFECTa RNAi Kits IDT - Integrated DNA Technologies

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WebApr 1, 2024 · to introduce Exo-FectTM siRNA/miRNA Transfection Kit (Cat #EXFT200A-1), a first-of-its-kind exosome transfection kit specially designed to load siRNA/miRNAs into EVs. It is based on a proprietary modified Cell-Penetrating Peptide (CPP) technology to complex siRNA/miRNAs and entry into EVs, followed by a depletion method to remove … WebMay 17, 2024 · System Biosciences' Exo-Fect siRNA/miRNA Transfection Kit gives you an exciting new way to load your small RNA cargo into extracellular vesicles (EVs). … fbb abflügeWebStemfect RNA Transfection Kit has been primary fully used to deliver RNA to human ESCs, dendritic cells, and retinal pigment epithelial cells. No need to serum-starve cells … hoolah singapore

"WebProduct Description: ExoFectin® sRNA-into-Exosome Kit (Chemical) is a unique blend of polymers designed for the delivery of small RNAs including miRNA and siRNA into … " - Exo-fecttm sirna/mirna transfection kit

Exo-fecttm sirna/mirna transfection kit

microRNA Extraction miRNA Isolation Kits QIAGEN

WebUnter Transfektion versteht man das Einbringen von genetischem Material, darunter DNA, RNA und Proteine, in eukaryotische Zellen zum Zwecke der Genexpression, des Gen-Knockdowns und vieler anderer Anwendungen. WebWe offer transfection reagents for DNA, siRNA, RNA, and protein delivery, providing a range of options to suit your transfection experiment: DNA delivery—Transient and stable transfection of DNA plasmids.DNA transfection is used to study gene function and regulation, mutational analysis and biochemical characterization of gene products, …

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WebThe Exo-Fect™ reagent enables the high efficiency transfection of nucleic acids directly into isolated exosomes. In less than one hour, create novel exosome delivery vehicles … WebDec 7, 2024 · Electroporation of miRNA mimics was performed under a high-voltage method (500 V for 10 ms) using 1.5 µL of miRNA mimics, 200 µL of EVs and 800 µL of electroporation buffer. Similarly, the Exo-Fect™ siRNA/miRNA Transfection Kit was used according to the manufacturer’s protocol, for targeted loading of miRNA mimics in GC …

WebRibobio co exo fect sirna mirna transfection reagent kit Exo Fect Sirna Mirna Transfection Reagent Kit, supplied by Ribobio co, used in various techniques. Bioz … Web2013: TransIT-X2® Dynamic Delivery System—Superior delivery of plasmid DNA and/or siRNA 2013: TransIT®-BrCa Transfection Reagent—The first breast cancer cell transfection reagent 2010: ®TransIT-PRO Transfection Kit—Large-scale, high yield protein production 2009: ®TransIT -2024 Transfection Reagent—For hard to transfect …

WebBioCat GmbH Im Neuenheimer Feld 584 69120 Heidelberg. Sales & Scientific Support phone +49 (0) 6221-714 15 16. New Orders [email protected] WebThe mirVana miRNA Probe Construction Kit includes reagents for preparation of in vitro transcription templates as well as reagents for in vitro transcription.For template preparation, all you need to supply is a short, inexpensive DNA oligonucleotide specific for the target of interest that also includes an 8 base sequence complementary to the 3' end of the T7 …

WebKnown for almost 30 years as premier providers of synthetic oligonucleotides, Dharmacon Reagents were the first to offer algorithm designed synthetic siRNA for RNAi, and the first to offer guide RNA for CRISPR-Cas9 gene editing applications. Dharmacon Reagents have you covered with a complete range of genomic solutions from single-gene ...

WebMar 6, 2024 · Exosomes are membrane-bound nanosized extracellular vesicles of 50-100nm diameters secreted by various cells including tumor cells and contain a wide variety of bioactive substances, including proteins, lipids, and nucleic acids,11which involve in multiple cell functions after the uptake of exosomes by target cells.12-14Besides, tumor … fbb airport assekuranzWebBelow is a protocol for the i.v. injection of nanoparticles for in vivo experiments. Dilute 100 µg of plasmid DNA or 60 µg of siRNA in 100 µL nuclease-free water and vortex gently. Add 100 µL of the diluted to a sterile tube containing 50 µL Transfection Reagent. Incubate for 15-20 min at room temperature. Add 10 µL of Transfection ... fbb ais คือWebOur miRNA isolation kits enable miRNA extraction from a variety of tissues and cells, including difficult-to-lyse tissues. The kits remove contaminants that could interfere with your downstream analyses and deliver superior yields compared to alternative methods. Products Sort Application Handling Sample type Starting material Format Instrument hoolah payment