Fitc solubility
WebApr 5, 2024 · Fluorescein isothiocyanate (FITC) was added to 20 mL of 1 mg/mL collagen carbonate buffer (0.1 M, pH 9.0), stirred at room temperature and crosslinked at 450 rpm for 18 h in the dark. The labeled collagen solution was centrifuged at 3000 rpm for 15 min, washed five times with carbonate buffer and stored at 4°C in the dark. WebApoptosis was quantified using FITC-conjugated annexin V (AV) according to the manufacturer’s protocol (Marine Biological Laboratory, Woods Hole, MA, USA). Annexin V-FITC staining in NK-92 cell lines was determined after 48 h in the absence or presence of different amounts of Aloe-emodin (9 mM stock solution in DMSO) ranging from 6.25 to …
Fitc solubility
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WebMar 25, 2013 · Fluorescein. Fluorescein is an organic dye also known as D & C Yellow No. 7 and many other names. A. von Baeyer first synthesized it in 1871 from phthalic anhydride and resorcinol and called it resorcinphthalein. It is not soluble in water or many organic solvents, but its disodium salt (fluorescein sodium, uranine, D & C Yellow No. 8) … WebSolubility: DMSO (10 mg/ml) Working concentration: 1 nM-10 µM Structure: BACKGROUND Geldanamycin is a benzoquinone ansamycin antibiotic produced by ... FITC -geldanamycin can be used to screen for new Hsp90 inhibitors by measuring its binding to Hsp90 through a fluorescent polarization assay. 1.
WebApplication: Fluorescein sodium salt is a sodium salt of fluorescein. CAS Number: 518-47-8. Molecular Weight: 376.27. Molecular Formula: C 20 H 10 O 5 • 2 Na. For Research … WebFITC (Fluorescein 5-isothiocyanate) is a fluorescence probe for the labeling of amines. FITC is a pH- and Cu 2+ -sensitive fluorescence dye [1] [2] . In Vitro. Annexin V-FITC/PI is employed to detect chondrocyte …
WebInvitrogen BODIPY FL dye is a bright, green-fluorescent dye with similar excitation and emission to fluorescein (FITC) or Invitrogen Alexa Fluor 488 dye. It has a high extinction coefficient and fluorescence quantum yield and is relatively insensitive to solvent polarity and pH change. ... In contrast to the highly water soluble fluorophores ... WebMar 22, 2015 · The solubility data for 2,3,4,5-tetrabromothiophene dissolved in binary ethanol + trichloromethane solvent mixtures is used to describe the preferential solvation …
WebDescription. A fluorescent labeling agent used to label antibodies, lectins, toxins, various proteins, and oligopeptides with fluorescein. FITC-antibody conjugates are useful in many applications, including flow cytometry and immunohistochemistry (excitation maximum = 492 nm; emission maximum = 520 nm). λ max: 492 ± 3 nm (pH 9).
WebSolubility Data for FITC. Solvent Max Conc. mg/mL Max Conc. mM; Solubility; DMSO: 38.94: 100: Preparing Stock Solutions for FITC. The … chicago old town folk musicWebFITC-dextrans have from 0.001-0.020 mol FITC per glucose unit. These low levels of substitution confer minimal charges to dextran, which is an essential requirement for permeability studies. Spectral data FITC-dextran has an excitation maximum of 493 nm and an emission maximum of 518 nm. Maximal intensity is observed at pH >8. Storage and … chicago old town apartments for rentWebMar 22, 2015 · The solubility data for 2,3,4,5-tetrabromothiophene dissolved in binary ethanol + trichloromethane solvent mixtures is used to describe the preferential solvation around the dissolved solute. View ... chicago old town shootingWebMar 10, 2016 · I am just quoting from an article " fluorescein, i.e. FITC-dextran, proved to be the most sensitive to pH, exhibiting more than a 95% decrease in fluorescence intensity as the pH decreased from 10 ... google earth pinguineWebFluorescein-5-isothiocyanate (FITC, Fluorescein isothiocyanate isomer I, 5-FITC) is a fluorescent probe capable of being conjugated to tissue and prot... Quality confirmed by … google earth pinjarraWebThe FITC solution is then diluted (1:50, 1.5:100, 1:100, 1:200) with PBS buffer by the students. 2.2. Bioconjugation. The protocol used for the modification of BSA with FITC is reported in literature 1 and is very simple: 1) Students are asked to prepare a 2 mg/ml BSA solution in 0.1 M sodium carbonate, pH 9. chicago old town newsWebFITC, also known as fluorescein 5-isothiocyanate, is a fluorescein derivative used in a variety of applications including flow cytometry. FITC reacts with amine groups to form conjugates with proteins for detection by various fluorescent-based application ... For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ... chicago old town neighborhood