Fluorescein arsenical hairpin binder
WebJun 2, 2024 · To better investigate this process, we developed sensitive assays that use the fluorescein arsenical dye FlAsH (fluorescein arsenical hairpin binder) to detect … WebOct 12, 2024 · The arsenical hairpin derivative of fluorescein, FlAsH, offers a highly specific labelling strategy of proteins via a small tetracysteine motif (FCM) which can be …
Fluorescein arsenical hairpin binder
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WebMar 15, 2015 · Using the established concept of FIAsH-EDT2 based binding with cysteine tagged biomolecules that result in fluorescence ‘ON or OFF’ system, we aim to establish a generic nanoswitch that can be used for multiple applications. Web北京大学医学部机构知识库(ir@pkuhsc)以发展机构知识能力和知识管理能力为目标，快速实现对本机构知识资产的收集、长期保存、合理传播利用，积极建设对知识内容进行捕获、转化、传播、利用和审计的能力，逐步建设包括知识内容分析、关系分析和能力审计在内的知识服务能力，开展综合知识 ...
Webon fluorophores which can attach to proteins in several sites positions: Fluorescein arsenical hairpin binder (FlAsH) contains, for instance, a biarsenical group that can bind to a tetracysteine motif Cys-Cys-Pro-Gly-Cys-Cys in proteins [22]. This label has been used to study orientational dynamics of a G-protein-coupled receptor in WebSep 23, 2010 · In this paper, we provide a general protocol for labeling proteins with the membrane-permeant fluorogenic biarsenical dye …
Web%0 Journal %B Nature Protocols %D 2010 %T Fluorescent labeling of tetracysteine-tagged proteins in intact cells %A Hoffmann, C. %A Gaietta, G. WebFeb 1, 2007 · However, the large size and spectral properties of FP impose certain limitations for their use. The recently developed Fluorescein Arsenical Hairpin (FlAsH/tetracysteine) binder technology...
WebOct 12, 2024 · Characterization of Fluorescein Arsenical Hairpin (FlAsH) as a Probe for Single-Molecule Fluorescence Spectroscopy Authors Dennis D Fernandes 1 2 , Jasbir …
WebJan 23, 2024 · Constructs were lentivirally transduced for subsequent CD36 labeling with fluorescein-arsenical hairpin-binder (FlAsH). Cell imaging was combined with V0/V1 … candy covered pretzels recipeWeb@journal { , title = {Fluorescent labeling of tetracysteine-tagged proteins in intact cells}, address = {}, booktitle = {Nature Protocols}, chapter = {}, edition ... candy covered sunflower seedsWebdyes, such as fluorescein arsenical hairpin binder (FlAsH) and resorufin arsenical hairpin binder (ReAsH) [16, 17]. FlAsH or ReAsH are prepared as ethane dithiol (EDT) … candy craft 1.12.2WebJan 23, 2024 · Methods Three human CD36 (hCD36) mutants were constructed via insertion of a tetracysteine motif at different positions within the extracellular domain. Constructs were lentivirally transduced for subsequent CD36 labeling with fluorescein-arsenical hairpin-binder (FlAsH). Cell imaging was combined with V-0/V-1 immunostaining and Western … fish technikWebThe receptors were C-terminally labeled with cyan fluorescent protein and with fluorescein arsenical hairpin binder at different sites in the third intracellular loop: N-terminally close to transmembrane domain V (I3-N), in the middle of the loop (I3-M), or C-terminally close to transmembrane domain VI (I3-C). fish technique diagramWebFor this study, the FlAsH (fluorescein arsenical hairpin-binder)-labelling procedure previously described Hoffmann et al. 75 was adjusted for 96-well plates. In brief, the cells … candy covered strawberriesWebfor subsequent CD36 labeling with fluorescein-arsenical hairpin-binder (FlAsH). Cell imag-ing was combined with V 0/V 1 immunostaining and Western blotting. Results Transduction of hCD36-wildtype and mutants yielded corresponding proteins in HL-1 cardi-omyocytes. Tetracysteine mutant-2 (hCD36-TC2) showed similar fatty acid uptake to wild … candy craft game