Nettet17. feb. 2024 · Lineweaver-Burk Plot. A graph of the double-reciprocal equation is also called a Lineweaver-Burk, 1/Vo vs 1/[S]. The y-intercept is 1/Vmax; the x-intercept is -1/KM; and the slope is KM/Vmax. Lineweaver-Burk graphs are particularly useful for analyzing how enzyme kinematics change in the presence of inhibitors, ... NettetUsually, Lineweaver–Burk plot (L–B plot) plot is applied to determine the kinetic parameters due to its simplicity [11]. However, we must pay attention to accuracy of …
Michaelis-Menten Kinetics - Chemistry LibreTexts
NettetLineweaver–Burk (L–B) plots, obtained from the urea–urease hydrolysis assay, were used to calculate Michaelis–Menten constant (Km) values for free and immobilized ureases. 2. The concentrations of urea were in the range of 0.14–1.4 mMand 0.062–0.312 mMfor UCG and free urease in PBS solution, respectively. 3. Nettetcan be explained in terms of specific metabolic processes (eg, participation of oxygenases, etc). At another level, the caloric value of various compounds can be … low key valentine\u0027s day ideas
Lineweaver-Burk Plot - an overview ScienceDirect Topics
NettetIn biochemistry, an Eadie–Hofstee diagram (more usually called an Eadie–Hofstee plot) is a graphical representation of the Michaelis–Menten equation in enzyme kinetics. It has been known by various different names, including Eadie plot, Hofstee plot and Augustinsson plot. NettetFigure 5.4.4: Line-Weaver Burk Plot of noncompetitive inhibition. Feedback inhibition is a normal biochemical process that makes use of noncompetitive inhibitors to control some enzymatic activity. In this process, the final product inhibits the enzyme that catalyzes the first step in a series of reactions. NettetLineweaver-Burk plot and Eadie-Hofstae methods; Competitive and non-competitive. 3 inhibition. Chapter 8. ... and an innovative vertical format for explaining reaction mechanisms, Fundamentals takes a modern approach: primary organization is by functional group, beginning with the simple (alkanes) ... lowkey ventures l.p