Nettet13. apr. 2024 · In the US and other western countries, acetaminophen (APAP) overdose is the most common cause of drug-induced liver injury (DILI), the leading reason for …
An efficient method to isolate and culture mouse Kupffer cells
Nettet18. mar. 2016 · To study the biological functions of liver sinusoidal endothelial cells (LSEC) and to identify their interplay with blood or liver cells, techniques allowing for the isolation and purification of LSEC have been developed over the last decades. The objective of the present review is to summarize and to compare the efficiency of … Nettet17. des. 2024 · Place perfusion buffer and digestion buffer sufficient for one digestion reaction in two separate 50 mL tubes in a 42°C water bath for at least 30 min prior to … forest forge ringwood
Mouse liver cell culture. I. Hepatocyte isolation - PubMed
Nettet3. jan. 2014 · The volume of the mouse liver is much smaller than that of the rat liver, which makes it much more difficult to isolate mouse HSCs (mHSCs) than rat HSCs. At … Nettet16. des. 2024 · SummaryAutomatic TranslationDecember 16th, 2024. Here we outline and demonstrate a protocol for primary mouse liver sinusoidal endothelial cell (LSEC) isolation. The protocol is based on liver collagenase perfusion, nonparenchymal cell purification by low-speed centrifugation, and CD146 magnetic bead selection. Nettet13. aug. 2024 · Protocol for Primary Mouse Hepatocyte Isolation. Primary hepatocytes are a vital tool in various biomedical research disciplines, serving as an ex vivo model for liver physiology. Obtaining high yields of viable primary mouse … dien thoai samsung a32