Phospho-akt1/2/3 ser473 cellular kit
WebMMP1 Antibody SKU: A... WebIn the case of AKT1/2, immunofluorescence is reduced following the augmentation of NP-427 concentrations (Figure 8A–D). Similar to the anterior cases, the Western blot showed a decrease in phospho-Thr308-Akt, phospho-Ser473-Akt, and total AKT at 24 h in cells treated with NP-427 at 0.5 and 0.75 mg/mL ( p < 0.05) ( Figure 8 E).
Phospho-akt1/2/3 ser473 cellular kit
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WebProduct Note This antibody is raised against human AKT1 phosphorylated at Ser473. Based on sequence homology, it is predicted to react with AKT2 and AKT3 when phosphorylated at the corresponding residues - AKT2 Ser474 and AKT3 Ser472. Predict Reactivity Zebrafish, Sheep, Bovine, Cat, Chicken, Rhesus Monkey (>80% identity) PROPERTIES TARGET LANCE Ultra phospho-AKT1/2/3 (Ser473) kits are designed for the detection of AKT1, AKT2, and AKT3 (phosphorylated at residue S473) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
WebApr 12, 2024 · Sex related differences have been observed in the progression of Alzheimer’s disease (AD) [1] and the prevalence of AD is greater in women than men in European and American patients [2, 3 ...
WebPhospho-Akt (Ser473) Kit For use with human, mouse, rat, and non-human primate cell lysates and tissue lysates. This package insert must be read in its entirety before using … WebThe Phospho-AKT3 (Ser473) kit is designed for the robust quantification of AKT3 modulation, phosphorylated on Ser473. Also known as protein kinase B (PKB), AKT is an oncogene playing a key role in controlling apoptosis, cell proliferation, transcription, cell migration, and glucose metabolism.
Web#IR172-668 / IR172-668,Phospho-AKT (Ser473) antibody. Products ... and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. ... Subsequent phosphorylation of both threonine residue 308 and serine ...
WebThe protocol is simple and straightforward, with just two incubation steps: - Cell stimulation by the target compounds - cAMP detection using HTRF reagents Product specifications Designed with signal performance in mind, the kit offers a tailored standard curve ideally suited to the study of Gi coupled GPCRs. Introduction to product validation how to stop being tired after lunchWebFeb 3, 2024 · Protein kinase B (AKT1) is hyper-activated in diverse human tumors. AKT1 is activated by phosphorylation at two key regulatory sites, Thr308 and Ser473. Active AKT1 phosphorylates many, perhaps hundreds, of downstream cellular targets in the cytosol and nucleus. AKT1 is well-known for phosphorylatin … how to stop being tired all the timeWebAKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of … reaction of iron and oxygenWebPhospho-AKT1/2/3 (Ser473) cellular kit HTRF® Kit Diabetes and metabolic disorders A portfolio to tackle in-depth diabetes and metabolic disorders studies Explore Phospho-AKT1/2/3 (Ser473) control lysate HTRF® Control lysate Phospho-AKT1/2/3 (Thr308) cellular kit HTRF® Kit Phospho-AKT1/2/3 (Thr308) control lysate HTRF® Control lysate Guides how to stop being too honestWebIn contrast to previous studies suggesting the impact of Ser473 and Ser824 phosphorylation on TRIM28 function, examined phospho-mutants and phospho-mimetics did not affect … how to stop being too niceWebThe Advanced Phospho-ERK assay kits are designed for the robust and highly sensitive quantification of ERK phosphorylation on Thr202/Tyr204 as an MAPK pathway readout. The simple mix-and-read protocol eliminates all wash steps for faster analysis and high quality output. The Total-ERK1/2 assay is compatible with the buffers from the Phospho or ... reaction of isocyanate with waterWebPhospho-AKT1 (Ser473) Antibody (700392) in ICC/IF Immunofluorescent analysis of AKT (pS473) was done on 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. how to stop being too agreeable