Slrp shape bridges collagen
WebbKnowing that almost all the members of the SLRP family are involved in collagen interaction as previously reviewed by Chen and Birk ... J.E.; Thomlinson, A.M. The … Webb25 juli 2024 · Removal of the disulfide bridge in EF-hand2, which is known to enhance Col(IV) binding by SPARC, ... collagen-binding epitopes and EF-hands of SPARC are …
Slrp shape bridges collagen
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WebbCollagen fibrils in extracellular matrices of connective tissues (tendon, cornea, etc.) are bridged and linked by the anionic glycosaminoglycans (AGAGs) of the small … Webb(A) Class I SLRPs. (B) Class II SLRPs. Red rectangles show the identified collagenbinding regions with high affinity, while the green rectangles correspond to collagen-binding …
Webb7 nov. 2011 · Changes in SLRP expression and modification in cornea, atherosclerotic plaque, joints, bone, tendons, and kidney may be associated with disease progression in … Webb14 aug. 1986 · Electron-microscopy revealed a D-periodic arrangement of glycosaminoglycan on the surfaces of collagen fibrils precipitated in the presence of …
Webb28 aug. 2011 · The observed G′ of this collagen mimetic system is similar to that typically observed for a collagen hydrogel formed from natural sources, such as rat-tail collagen, … WebbDecorin, fibromodulin and lumican are small leucine-rich repeat proteoglycans (SLRPs) which interact with the surface of collagen fibrils. Together with other molecules they form a coat on the fibril surface which could impede the access to collagenolytic proteinases. To address this hypothesis, fibrils of type I or type II collagen were formed in vitro and …
Webbshape tissue-specific function. The extracellular matrix is mainly composed of glycoproteins, proteoglycans and collagens. SLRPs, with their multivalent binding …
WebbFragmentation of SLRP's in facet joint articular cartilage ... Real-time PCR showed highest mRNA expression of lumican and collagen I. Collagen I and proteoglycan … sharp congelateurs armoire sharp sjsc11cmxwfWebbPurpose: Proteoglycans, especially small leucine rich proteoglycans (SLRPs), play major roles in facilitating the development and regulation of collagen fibers and other … sharp conference series projector xg-ph50xInteraction analysis between OMD (or muteins) and collagen was analyzed using SPR in a Biacore T200 instrument (GE Healthcare). A CM5 Biacore sensor chip (GE Healthcare) was used and activated by a treatment with N-hydroxysuccinimide/N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide hydrochloride, … Visa mer Expression and purification of human full-length OMD and muteins were performed as described previously11. Human OMD (without residues 1–20 corresponding to the signal peptide) with a C-terminal FLAG-tag was inserted into … Visa mer SEC-MALS was performed at room temperature and using PBS buffer (pH 7.4). Proteins concentrated at 25 µM were loaded on a Hiload 10/300 Superdex 200 pg column (GE … Visa mer Data collection was carried out at beamline AR-NE3A at the Photon Factory (Tsukuba, Japan) at 1.000 Å and 100 K. Diffraction images were processed with the program … Visa mer Purified OMD (9.9 mg/ml) was screened in an Oryx8 automatic protein crystallization system (Douglas Instrument) with commercially available … Visa mer sharpconfig