WebJun 29, 2024 · An alternative way of stretching DNA in nanofluidic channels was presented by Matsuoka et al.28 They used PDMS to make nanochannels where DNA and chromatin can be fully stretched. Since PDMS is a soft material it is possible to vary the size of the channels by varying the strain of the material. WebFeb 1, 2024 · Towards the end of the translocation, DNA segments faced a weaker retarding force due to the lower amount of DNA residing outside the entrance, thereby yielding higher translocation velocities. The weak forces involved in the translocation process did not stretch the DNA during translocation but allowed folds and knots to persist inside the slit.
Translocation of DNA through Ultrathin Nanoslits - Yang - 2024
Websteady stretching of DNA molecules of varying lengths in a constant electric field. Our data support the theory of hydrodynamic equivalence proposed by Long and Viovy (Long et al., 1996a,b), which purports that DNA in an electric field will stretch in the same manner as if it were deformed by hydrodynamic flow with velocity mE where m WebAug 5, 2011 · For instance, Jo et al. showed that DNA stretch is possible in relatively large (100 nm) nanoslits by using low-ionic-strength buffers. And Cross et al. ( 2007 ) … summit 2801 camshaft
Stretching of Tethered DNA in Nanoslits - ACS Macro Letters (ACS
WebExternal forces and confinement are two fundamental and complementary approaches for biopolymer stretching. By employing micro- and nanofluidics, we study the force–extension dynamics by simultaneously applying external forces and confinement to single-DNA molecules. In particular, we apply external electric fields to stretch single DNA molecules … WebApr 17, 2007 · The nanoslit geometry, however, physically confines polymer molecules to two spatial dimensions; further reduction in configurational entropy resulting in axially stretched molecules arises spontaneously and appears to be electrostatically mediated. WebAug 5, 2011 · Single well-designed and controlled nanochannels are ideal physical modeling systems to study fluidics in a precise manner. For instance, Jo et al. ( 2007) showed that DNA stretch is possible in relatively large (100 nm) … summit 26 mystery ranch